MA5-11803

antibody from Invitrogen Antibodies

Targeting: IVL

Antibody data

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Validation data

Product number: MA5-11803 - Provider product page
Provider: Invitrogen Antibodies
Product name: Involucrin Monoclonal Antibody (SY5)
Antibody type: Monoclonal
Antigen: Other
Description: MA5-11803 targets Involucrin in ELISA, IF, IHC (P), IP, and WB applications and shows reactivity with Canine, Human, Non-human primate, Porcine, and Rat samples.
Antibody clone number: SY5
Concentration: 0.2 mg/mL

IVL protein structure - MA5-11803 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 3 Late passage SBK stratify into an epidermis with increased numbers of cornified cells present in the subcorneal layers. Hematoxylin and eosin stain (A, B; size of stratum corneum highlighted as 'SC') and immunofluorescence stain (C, D; green, keratin 14; red, involucrin; blue, DAPI) of organotypic raft cultures derived from early and late passage keratinocytes. Arrow in B indicates cornified cells in the sub-corneal layers. DAPI image presented in black and white to better distinguish the nuclei, and the red dotted line delineates where the epidermis begins. Nuclei below the line are from the 3T3 fibroblasts seeded in the collagen rafts. sc = stratum corneum, sg = stratum granulosum, scale bar = 100muM for both H&E and IF photos, seen in the upper left corner of each figure.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 IFN kappa does not regulate NHEK differentiation. (a) Western blot assay to detect IFN kappa , IVL, KRT10, and beta -actin in undifferentiated (UD) and differentiated (D) NHEK in the presence and absence of rhIFN kappa . (b) Western blot assay to detect IFN kappa , IVL, KRT10, and beta -actin in IFN kappa -silenced undifferentiated (UD) and differentiated (D) NHEK. One of three independent experiments is presented.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 All-trans retinoic acid (ATRA) treatment. Seven-day-old SCESM spheroids were treated with 10 muM ATRA for 5 days. ( a ) Normalized fluorescence intensities of selected surface stem markers and corresponding flow cytometry visible light and fluorescent photos are presented. Normalized mean values +- SE ( n >= 5, N >= 2000) are shown. ( b ) Relative gene expression of two differentiation markers, CDH1 and IVL , after 5 days of ATRA treatment are displayed. Normalized mean values +- SE ( n >= 5) are presented. ( c ) IVL protein expression was assessed after 5 days of ATRA treatment by Western blot analysis. Western blot membrane photo showing IVL and Ponceau staining and a graph depicting normalized IVL protein expression analysis from the Western blot membrane, (using Image Lab software) are presented ( n >= 3). (* p < 0.05, **** p < 0.0001 vs. control - ATRA untreated group)--control/ATRA untreated group; +: ATRA treated group.