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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF5884 - Provider product page
- Provider
- R&D Systems
- Product name
- Human DCC Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human DCC in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ICAM-1, rhICAM-2, rhICAM-3, rhMadCAM-1, rhPECAM-1, rhVCAM-1, and rhCEACAM-1 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P43146- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human DCC by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human DCC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5884) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DCC at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
