Antibody data
- Antibody Data
- Antigen structure
- References [43]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 10231-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#10231-1-AP, RRID:AB_2193323
- Product name
- SMAD4 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated SMAD4 antibody (Cat. #10231-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references The Jumonji Domain-Containing Histone Demethylase Homolog 1D/lysine Demethylase 7A (JHDM1D/KDM7A) Is an Epigenetic Activator of RHOJ Transcription in Breast Cancer Cells.
Pilose Antler Peptide-3.2KD Ameliorates Adriamycin-Induced Myocardial Injury Through TGF-β/SMAD Signaling Pathway.
FOXM1-induced miR-552 expression contributes to pancreatic cancer progression by targeting multiple tumor suppressor genes.
Huangqi Shengmai Yin Ameliorates Myocardial Fibrosis by Activating Sirtuin3 and Inhibiting TGF-β/Smad Pathway.
SMAD4 Feedback Activates the Canonical TGF-β Family Signaling Pathways.
Smad4 deficiency substitutes Cdkn2b but not Cdkn2a downregulation in pancreatic cancer following induction of genetic events in adult mice.
MiR-664-3p suppresses osteoblast differentiation and impairs bone formation via targeting Smad4 and Osterix.
SMAD4 Inhibits Granulosa Cell Apoptosis via the miR-183-96-182 Cluster and FoxO1 Axis.
A mesenchymal-like subpopulation in non-neuroendocrine SCLC contributes to metastasis.
SEPHS1 promotes SMAD2/3/4 expression and hepatocellular carcinoma cells invasion.
Comparison of anticarcinogenic properties of Viburnum opulus and its active compound p-coumaric acid on human colorectal carcinoma.
Novel Alternatively Spliced Variants of Smad4 Expressed in TGF-β-Induced EMT Regulating Proliferation and Migration of A549 Cells.
Trimetazidine Inhibits Renal Tubular Epithelial Cells to Mesenchymal Transition in Diabetic Rats via Upregulation of Sirt1.
Deubiquitinase USP13 promotes extracellular matrix expression by stabilizing Smad4 in lung fibroblast cells.
KDM6A suppresses hepatocellular carcinoma cell proliferation by negatively regulating the TGF-β/SMAD signaling pathway.
Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis.
Small airway remodeling in diabetic and smoking chronic obstructive pulmonary disease patients.
Co-transplantation with adipose-derived cells to improve parathyroid transplantation in a mice model.
Si-Miao-Yong-An decoction attenuates cardiac fibrosis via suppressing TGF-β1 pathway and interfering with MMP-TIMPs expression.
Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2.
β2-microglobulin as a biomarker of pulmonary fibrosis development in COPD patients.
Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway.
Overexpression of matrix metalloproteinase-9 in breast cancer cell lines remarkably increases the cell malignancy largely via activation of transforming growth factor beta/SMAD signalling.
Oviductus Ranae protein hydrolyzate prevents menopausal osteoporosis by regulating TGFβ/BMP2 signaling.
The role and mechanism of transforming growth factor beta 3 in human myocardial infarction-induced myocardial fibrosis.
miR-93-5p targets Smad7 to regulate the transforming growth factor-β1/Smad3 pathway and mediate fibrosis in drug-resistant prolactinoma.
Myostatin/SMAD4 signaling-mediated regulation of miR-124-3p represses glucocorticoid receptor expression and inhibits adipocyte differentiation.
Inhibition of Skp1-Cullin-F-box complexes during bovine oocyte maturation and preimplantation development leads to delayed development of embryos†.
Transcription coactivator Cited1 acts as an inducer of trophoblast-like state from mouse embryonic stem cells through the activation of BMP signaling.
Inhibition of lysyl oxidase expression by dextran sulfate affects invasion and migration of gastric cancer cells.
Role of TGF-β1/Smad3-mediated fibrosis in drug resistance mechanism of prolactinoma.
MicroRNA-140 Inhibits the Epithelial-Mesenchymal Transition and Metastasis in Colorectal Cancer.
SIRT7 suppresses the epithelial-to-mesenchymal transition in oral squamous cell carcinoma metastasis by promoting SMAD4 deacetylation.
Inhibiting transforming growth factor-β signaling regulates in vitro maintenance and differentiation of bovine bone marrow mesenchymal stem cells.
Gene Correction Reverses Ciliopathy and Photoreceptor Loss in iPSC-Derived Retinal Organoids from Retinitis Pigmentosa Patients.
TGFβ1 Promotes Gemcitabine Resistance through Regulating the LncRNA-LET/NF90/miR-145 Signaling Axis in Bladder Cancer.
Lnc-ATB contributes to gastric cancer growth through a MiR-141-3p/TGFβ2 feedback loop.
Decrease of FSTL1-BMP4-Smad signaling predicts poor prognosis in lung adenocarcinoma but not in squamous cell carcinoma.
Prognostic Value of SMAD4 in Pancreatic Cancer: A Meta-Analysis.
Let‑7a suppresses cell proliferation via the TGF‑β/SMAD signaling pathway in cervical cancer.
Anti-fibrotic effects of neferine on carbon tetrachloride-induced hepatic fibrosis in mice.
Biomarkers of TGF-β signaling pathway and prognosis of pancreatic cancer.
Aberrant elevated microRNA-146a in dendritic cells (DC) induced by human pancreatic cancer cell line BxPC-3-conditioned medium inhibits DC maturation and activation.
Zhang Z, Chen B, Zhu Y, Zhang T, Zhang X, Yuan Y, Xu Y
Frontiers in cell and developmental biology 2021;9:664375
Frontiers in cell and developmental biology 2021;9:664375
Pilose Antler Peptide-3.2KD Ameliorates Adriamycin-Induced Myocardial Injury Through TGF-β/SMAD Signaling Pathway.
Xu Y, Qu X, Zhou J, Lv G, Han D, Liu J, Liu Y, Chen Y, Qu P, Huang X
Frontiers in cardiovascular medicine 2021;8:659643
Frontiers in cardiovascular medicine 2021;8:659643
FOXM1-induced miR-552 expression contributes to pancreatic cancer progression by targeting multiple tumor suppressor genes.
Wang X, Dou N, Wang J, Zhang Y, Li Y, Gao Y
International journal of biological sciences 2021;17(4):915-925
International journal of biological sciences 2021;17(4):915-925
Huangqi Shengmai Yin Ameliorates Myocardial Fibrosis by Activating Sirtuin3 and Inhibiting TGF-β/Smad Pathway.
Pan J, Cao Z, Fang C, Lei Y, Sun J, Huang X, Han D
Frontiers in pharmacology 2021;12:722530
Frontiers in pharmacology 2021;12:722530
SMAD4 Feedback Activates the Canonical TGF-β Family Signaling Pathways.
Liu L, Li Q, Yang L, Li Q, Du X
International journal of molecular sciences 2021 Sep 16;22(18)
International journal of molecular sciences 2021 Sep 16;22(18)
Smad4 deficiency substitutes Cdkn2b but not Cdkn2a downregulation in pancreatic cancer following induction of genetic events in adult mice.
Jia X, Sun B, Tu Q, Qi H, Li L, Liu X, Yan L, Dai H, Kong Q, Tang C, Zhao X
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] 2021 Mar;21(2):418-427
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] 2021 Mar;21(2):418-427
MiR-664-3p suppresses osteoblast differentiation and impairs bone formation via targeting Smad4 and Osterix.
Xu Y, Jin Y, Hong F, Ma Y, Yang J, Tang Y, Zhu Z, Wu J, Bao Q, Li L, Yao B, Li D, Ma C
Journal of cellular and molecular medicine 2021 Jun;25(11):5025-5037
Journal of cellular and molecular medicine 2021 Jun;25(11):5025-5037
SMAD4 Inhibits Granulosa Cell Apoptosis via the miR-183-96-182 Cluster and FoxO1 Axis.
Yao W, Wang S, Du X, Lin C, Zhang J, Pan Z, Li Q
Reproductive sciences (Thousand Oaks, Calif.) 2021 Jul 21;
Reproductive sciences (Thousand Oaks, Calif.) 2021 Jul 21;
A mesenchymal-like subpopulation in non-neuroendocrine SCLC contributes to metastasis.
Jin Y, Xiao T, Feng Y, Yang J, Guo C, Hu L, Ji H
Journal of genetics and genomics = Yi chuan xue bao 2021 Jul 20;48(7):571-581
Journal of genetics and genomics = Yi chuan xue bao 2021 Jul 20;48(7):571-581
SEPHS1 promotes SMAD2/3/4 expression and hepatocellular carcinoma cells invasion.
Yang S, Zhang H, Yang H, Zhang J, Wang J, Luo T, Jiang Y, Hua H
Experimental hematology & oncology 2021 Feb 23;10(1):17
Experimental hematology & oncology 2021 Feb 23;10(1):17
Comparison of anticarcinogenic properties of Viburnum opulus and its active compound p-coumaric acid on human colorectal carcinoma.
Karakurt S, AbuŞoĞlu G, Arituluk ZC
Turkish journal of biology = Turk biyoloji dergisi 2020;44(5):252-263
Turkish journal of biology = Turk biyoloji dergisi 2020;44(5):252-263
Novel Alternatively Spliced Variants of Smad4 Expressed in TGF-β-Induced EMT Regulating Proliferation and Migration of A549 Cells.
Wan R, Xu X, Ma L, Chen Y, Tang L, Feng J
OncoTargets and therapy 2020;13:2203-2213
OncoTargets and therapy 2020;13:2203-2213
Trimetazidine Inhibits Renal Tubular Epithelial Cells to Mesenchymal Transition in Diabetic Rats via Upregulation of Sirt1.
Yang Y, Wang Y, He Z, Liu Y, Chen C, Wang Y, Wang DW, Wang H
Frontiers in pharmacology 2020;11:1136
Frontiers in pharmacology 2020;11:1136
Deubiquitinase USP13 promotes extracellular matrix expression by stabilizing Smad4 in lung fibroblast cells.
Liao X, Li Y, Liu J, Zhang Y, Tan J, Kass DJ, Rojas M, Mallampalli RK, Zhao J, Zhao Y
Translational research : the journal of laboratory and clinical medicine 2020 Sep;223:15-24
Translational research : the journal of laboratory and clinical medicine 2020 Sep;223:15-24
KDM6A suppresses hepatocellular carcinoma cell proliferation by negatively regulating the TGF-β/SMAD signaling pathway.
Li Y, Yang J, Zhang X, Liu H, Guo J
Experimental and therapeutic medicine 2020 Sep;20(3):2774-2782
Experimental and therapeutic medicine 2020 Sep;20(3):2774-2782
Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis.
Wang X, Liao X, Zhang Y, Wei L, Pang Y
Scientific reports 2020 Sep 2;10(1):14476
Scientific reports 2020 Sep 2;10(1):14476
Small airway remodeling in diabetic and smoking chronic obstructive pulmonary disease patients.
Wu N, Wu Z, Sun J, Yan M, Wang B, Du X, Liu Y
Aging 2020 May 5;12(9):7927-7944
Aging 2020 May 5;12(9):7927-7944
Co-transplantation with adipose-derived cells to improve parathyroid transplantation in a mice model.
Cui Q, Zhang D, Kong D, Tang J, Liao X, Yang Q, Ren J, Gong Y, Wu G
Stem cell research & therapy 2020 May 26;11(1):200
Stem cell research & therapy 2020 May 26;11(1):200
Si-Miao-Yong-An decoction attenuates cardiac fibrosis via suppressing TGF-β1 pathway and interfering with MMP-TIMPs expression.
Su C, Wang Q, Luo H, Jiao W, Tang J, Li L, Tian L, Chen X, Liu B, Yu X, Li S, Guo S, Wang W
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2020 Jul;127:110132
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2020 Jul;127:110132
Transactivation of miR-202-5p by Steroidogenic Factor 1 (SF1) Induces Apoptosis in Goat Granulosa Cells by Targeting TGFβR2.
Ding Q, Jin M, Wang Y, Liu J, Kalds P, Wang Y, Yang Y, Wang X, Chen Y
Cells 2020 Feb 14;9(2)
Cells 2020 Feb 14;9(2)
β2-microglobulin as a biomarker of pulmonary fibrosis development in COPD patients.
Wu Z, Yan M, Zhang M, Wu N, Ma G, Wang B, Fan Y, Du X, Ding C, Liu Y
Aging 2020 Dec 21;13(1):1251-1263
Aging 2020 Dec 21;13(1):1251-1263
Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway.
Sha L, Ma D, Chen C
Aging 2020 Dec 13;12(23):23598-23608
Aging 2020 Dec 13;12(23):23598-23608
Overexpression of matrix metalloproteinase-9 in breast cancer cell lines remarkably increases the cell malignancy largely via activation of transforming growth factor beta/SMAD signalling.
Dong H, Diao H, Zhao Y, Xu H, Pei S, Gao J, Wang J, Hussain T, Zhao D, Zhou X, Lin D
Cell proliferation 2019 Sep;52(5):e12633
Cell proliferation 2019 Sep;52(5):e12633
Oviductus Ranae protein hydrolyzate prevents menopausal osteoporosis by regulating TGFβ/BMP2 signaling.
Li X, Sui X, Yang Q, Li Y, Li N, Shi X, Han D, Li Y, Huang X, Yu P, Qu X
Archives of gynecology and obstetrics 2019 Mar;299(3):873-882
Archives of gynecology and obstetrics 2019 Mar;299(3):873-882
The role and mechanism of transforming growth factor beta 3 in human myocardial infarction-induced myocardial fibrosis.
Xue K, Zhang J, Li C, Li J, Wang C, Zhang Q, Chen X, Yu X, Sun L, Yu X
Journal of cellular and molecular medicine 2019 Jun;23(6):4229-4243
Journal of cellular and molecular medicine 2019 Jun;23(6):4229-4243
miR-93-5p targets Smad7 to regulate the transforming growth factor-β1/Smad3 pathway and mediate fibrosis in drug-resistant prolactinoma.
Hu B, Mao Z, Du Q, Jiang X, Wang Z, Xiao Z, Zhu D, Wang X, Zhu Y, Wang H
Brain research bulletin 2019 Jul;149:21-31
Brain research bulletin 2019 Jul;149:21-31
Myostatin/SMAD4 signaling-mediated regulation of miR-124-3p represses glucocorticoid receptor expression and inhibits adipocyte differentiation.
Liu K, Zhang X, Wei W, Liu X, Tian Y, Han H, Zhang L, Wu W, Chen J
American journal of physiology. Endocrinology and metabolism 2019 Apr 1;316(4):E635-E645
American journal of physiology. Endocrinology and metabolism 2019 Apr 1;316(4):E635-E645
Inhibition of Skp1-Cullin-F-box complexes during bovine oocyte maturation and preimplantation development leads to delayed development of embryos†.
Kinterova V, Kanka J, Petruskova V, Toralova T
Biology of reproduction 2019 Apr 1;100(4):896-906
Biology of reproduction 2019 Apr 1;100(4):896-906
Transcription coactivator Cited1 acts as an inducer of trophoblast-like state from mouse embryonic stem cells through the activation of BMP signaling.
Xu Y, Luo X, Fang Z, Zheng X, Zeng Y, Zhu C, Gu J, Tang F, Hu Y, Hu G, Jin Y, Li H
Cell death & disease 2018 Sep 11;9(9):924
Cell death & disease 2018 Sep 11;9(9):924
Inhibition of lysyl oxidase expression by dextran sulfate affects invasion and migration of gastric cancer cells.
Xu Y, Wang X, Huang Y, Ma Y, Jin X, Wang H, Wang J
International journal of molecular medicine 2018 Nov;42(5):2737-2749
International journal of molecular medicine 2018 Nov;42(5):2737-2749
Role of TGF-β1/Smad3-mediated fibrosis in drug resistance mechanism of prolactinoma.
Hu B, Mao Z, Jiang X, He D, Wang Z, Wang X, Zhu Y, Wang H
Brain research 2018 Nov 1;1698:204-212
Brain research 2018 Nov 1;1698:204-212
MicroRNA-140 Inhibits the Epithelial-Mesenchymal Transition and Metastasis in Colorectal Cancer.
Li J, Zou K, Yu L, Zhao W, Lu Y, Mao J, Wang B, Wang L, Fan S, Song B, Li L
Molecular therapy. Nucleic acids 2018 Mar 2;10:426-437
Molecular therapy. Nucleic acids 2018 Mar 2;10:426-437
SIRT7 suppresses the epithelial-to-mesenchymal transition in oral squamous cell carcinoma metastasis by promoting SMAD4 deacetylation.
Li W, Zhu D, Qin S
Journal of experimental & clinical cancer research : CR 2018 Jul 13;37(1):148
Journal of experimental & clinical cancer research : CR 2018 Jul 13;37(1):148
Inhibiting transforming growth factor-β signaling regulates in vitro maintenance and differentiation of bovine bone marrow mesenchymal stem cells.
Zhao XX, An XL, Zhu XC, Jiang Y, Zhai YH, Zhang S, Cai NN, Tang B, Li ZY, Zhang XM
Journal of experimental zoology. Part B, Molecular and developmental evolution 2018 Dec;330(8):406-416
Journal of experimental zoology. Part B, Molecular and developmental evolution 2018 Dec;330(8):406-416
Gene Correction Reverses Ciliopathy and Photoreceptor Loss in iPSC-Derived Retinal Organoids from Retinitis Pigmentosa Patients.
Deng WL, Gao ML, Lei XL, Lv JN, Zhao H, He KW, Xia XX, Li LY, Chen YC, Li YP, Pan D, Xue T, Jin ZB
Stem cell reports 2018 Apr 10;10(4):1267-1281
Stem cell reports 2018 Apr 10;10(4):1267-1281
TGFβ1 Promotes Gemcitabine Resistance through Regulating the LncRNA-LET/NF90/miR-145 Signaling Axis in Bladder Cancer.
Zhuang J, Shen L, Yang L, Huang X, Lu Q, Cui Y, Zheng X, Zhao X, Zhang D, Huang R, Guo H, Yan J
Theranostics 2017;7(12):3053-3067
Theranostics 2017;7(12):3053-3067
Lnc-ATB contributes to gastric cancer growth through a MiR-141-3p/TGFβ2 feedback loop.
Lei K, Liang X, Gao Y, Xu B, Xu Y, Li Y, Tao Y, Shi W, Liu J
Biochemical and biophysical research communications 2017 Mar 11;484(3):514-521
Biochemical and biophysical research communications 2017 Mar 11;484(3):514-521
Decrease of FSTL1-BMP4-Smad signaling predicts poor prognosis in lung adenocarcinoma but not in squamous cell carcinoma.
Chiou J, Su CY, Jan YH, Yang CJ, Huang MS, Yu YL, Hsiao M
Scientific reports 2017 Aug 29;7(1):9830
Scientific reports 2017 Aug 29;7(1):9830
Prognostic Value of SMAD4 in Pancreatic Cancer: A Meta-Analysis.
Shugang X, Hongfa Y, Jianpeng L, Xu Z, Jingqi F, Xiangxiang L, Wei L
Translational oncology 2016 Feb;9(1):1-7
Translational oncology 2016 Feb;9(1):1-7
Let‑7a suppresses cell proliferation via the TGF‑β/SMAD signaling pathway in cervical cancer.
Wu T, Chen X, Peng R, Liu H, Yin P, Peng H, Zhou Y, Sun Y, Wen L, Yi H, Li A, Zhang Z
Oncology reports 2016 Dec;36(6):3275-3282
Oncology reports 2016 Dec;36(6):3275-3282
Anti-fibrotic effects of neferine on carbon tetrachloride-induced hepatic fibrosis in mice.
Chen MS, Zhang JH, Wang JL, Gao L, Chen XX, Xiao JH
The American journal of Chinese medicine 2015;43(2):231-40
The American journal of Chinese medicine 2015;43(2):231-40
Biomarkers of TGF-β signaling pathway and prognosis of pancreatic cancer.
Javle M, Li Y, Tan D, Dong X, Chang P, Kar S, Li D
PloS one 2014;9(1):e85942
PloS one 2014;9(1):e85942
Aberrant elevated microRNA-146a in dendritic cells (DC) induced by human pancreatic cancer cell line BxPC-3-conditioned medium inhibits DC maturation and activation.
Du J, Wang J, Tan G, Cai Z, Zhang L, Tang B, Wang Z
Medical oncology (Northwood, London, England) 2012 Dec;29(4):2814-23
Medical oncology (Northwood, London, England) 2012 Dec;29(4):2814-23
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 10231-1-AP(SMAD4 antibody) at dilution of 1:600
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The SMAD4 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human SMAD4. This antibody recognizes human, mouse antigen. The SMAD4 antibody has been validated for the following applications: ELISA, WB, IHC analysis.