308102

antibody from BioLegend

Targeting: PRF1 HPLH2, P1, PFP

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

308102

Provider

BioLegend

Proper citation

BioLegend Cat#308102, RRID:AB_314700

Product name

Purified anti-human Perforin

Antibody type

Monoclonal

Antigen

Purified granules from the human lymphoma cell line

Description

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.

Reactivity

Human, Bovine, Feline

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

The antibody solution should be stored undiluted at 4°C.

References

Submitted references

Different NK cell-activating receptors preferentially recruit Rab27a or Munc13-4 to perforin-containing granules for cytotoxicity.

Wood SM, Meeths M, Chiang SC, Bechensteen AG, Boelens JJ, Heilmann C, Horiuchi H, Rosthøj S, Rutynowska O, Winiarski J, Stow JL, Nordenskjöld M, Henter JI, Ljunggren HG, Bryceson YT
Blood 2009 Nov 5;114(19):4117-27

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Whole blood lymphocytes stainedintracellularly with dG9 FITC

Conjugate

Unconjugated