Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-96046 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD150 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ASYGTGGRMM NCPKILRQLG SKVLLPLTYE RINKSMNKSI HIVVTMAKSL ENSVENKIVS LDPSEAGPPR YLGDRYKFYL ENLTLGIRES RKEDEGWYLM TLEKNVSVQR FCLQLRLYEQ VSTPEIKVLN KTQENGTCTL ILGCTVEKGD HVAYSWSEKA GTHPLNPANS SHLLSLTLGP QHADNIYICT VSNPISNNSQ TFSPWPGCRT DPSETKP; Positive Samples: Jurkat, Mouse liver; Cellular Location: Cell membrane, Single-pass type I membrane protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.62 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references SLAMF1 Promotes Methotrexate Resistance via Activating Autophagy in Choriocarcinoma Cells.
Shi D, Zhang Y, Tian Y
Cancer management and research 2020;12:13427-13436
Cancer management and research 2020;12:13427-13436
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using SLAMF1 Polyclonal antibody (Product # PA5-96046) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 5s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CD150 in extracts of various cell lines using CD150 Polyclonal Antibody (Product # PA5-96046) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 SLAMF1 expression is increased in MTX-resistant choriocarcinoma cells. ( A ). The mass spectrum of SLAMF1 unique peptide (SLENSVENK) identified by TMT labeling and LC-MS/MS. ( B ). Relative expression of SLAMF1 protein in JEG3 and its MTX-resistant subline JEG3/MTX. *P
