Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB1642 - Provider product page
- Provider
- R&D Systems
- Product name
- Human SLAM/CD150 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human SLAM/CD150 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q13291- Isotype
- IgG
- Antibody clone number
- 542301
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human SLAM/CD150 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with 10 ng/mL PMA and 200 ng/mL ionomycin for 48 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB1642), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SLAM/CD150 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of SLAM/CD150 in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB1642, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).
