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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- UM800096 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM800096, RRID:AB_2629205
- Product name
- Troponin C (TNNC1) mouse monoclonal antibody,clone UMAB204
- Antibody type
- Monoclonal
- Description
- Troponin C (TNNC1) mouse monoclonal antibody,clone UMAB204
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TNNC1
- Isotype
- IgG
- Antibody clone number
- UMAB204
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (15ug) from 9 Human tissue by using anti-TNNC1 monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen).(1:500) Dilution: 1:500
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of cell lysates (35ug) from 8 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: MCF7, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12). Diluation: 1:500.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-TNNC1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800096)(1:200)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-TNNC1 mouse monoclonal antibody (UM800096). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100)
- Validation comment
- 10K-CHIP
