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LearnMA1-41314
antibody from Invitrogen Antibodies
Targeting: NFKB1
KBF1, NF-kappaB, NF-kB1, NFkappaB, NFKB-p50, p105, p50
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- MA1-41314 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFkB p50 Monoclonal Antibody (2J10D7)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody is predicted to react with mouse and rat samples based on sequence homology. Suggested positive control: antigen standard for NFKB1 (transient overexpression lysate), Hela whole cell extract, HeLa cell lysate.
- Reactivity
- Human, Rat, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2J10D7
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFkB p50 (Fig. a) was performed by loading 20 µg of HeLa Control (lane 1), HeLa NFkB p50 knockout (lane 2) whole cell extracts. NFkB p50 was detected at 50 kDa using NFkB p50 mouse monoclonal Antibody (Product # MA1-41314, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody HRP conjugate (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal in CRISPR mediated knockout (KO) confirms that antibody is specific to NFkB p50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-431 (Lane 1), U-87 MG (Lane 2), A549 (Lane 3), Jurkat (Lane 4), Raji (Lane 5), K-562 (Lane 6) and HeLa (Lane 7). The blot was probed with Anti-NFkB p50 Monoclonal Antibody (Product # MA1-41314, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 50 kDa band corresponding to NFkB p50 was observed across the cell lines tested with an additional band at 105 kDa which is known to be the precursor of NFkB p50 called p105.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFkB p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide. Samples were incubated in NFkB p50 monoclonal antibody (Product # MA1-41314) using a dilution of 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NFkB p50 in human breast tumor tissue. Samples were incubated in NFkB p50 monoclonal antibody (Product # MA1-41314) using a dilution of 5 µg/mL. Isotype control antibody (top) and p50 antibody (bottom).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of NFkB p50 in U2-OS cells. Samples were incubated in NFkB p50 monoclonal antibody (Product # MA1-41314) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to DyLight 650.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of NFkB p50 in HeLa cells. Samples were incubated in NFkB p50 monoclonal antibody (Product # MA1-41314) using a dilution of 2.5 µg/mL for 30 minutes. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Both antibodies were conjugated to Dylight 650.
