310939

antibody from BioLegend

Targeting: CD69 CLEC2C

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Recommended by provider)

Antibody Data

Product number

310939

Provider

BioLegend

Proper citation

BioLegend Cat#310939, RRID:AB_2562827

Product name

Purified anti-human CD69 (MaxPar® Ready)

Antibody type

Monoclonal

Antigen

0

Description

CD69 is a 27-33 kD type II transmembrane protein also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3. It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4⁺ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.

Reactivity

Human, Porcine

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

FN50

Vial size

100 µg

Concentration

1 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human PBMCs were incubated for 6 hours in media alone (bottom) or with PMA and Ionomycin (top) in the presence of monensin and brefeldin A. Cells were then fixed, permeabilized, and stained with 154Sm-anti-CD45 (HI30) and 162Dy-anti-CD69 (FN50).

Conjugate

Unconjugated