MAB5747

antibody from Abnova Corporation

Targeting: CD69 CLEC2C

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB5747

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB5747, RRID:AB_10558760

Product name

Cd69 monoclonal antibody, clone H1.2F3 (PE/Cy7)

Antibody type

Monoclonal

Description

Hamster monoclonal antibody raised against native Cd69.

Isotype

IgG

Antibody clone number

H1.2F3

Storage

Store in the dark at 4°C. Do not freeze.Avoid prolonged exposure to light.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The mouse CD69 gene. Structure, expression, and mapping to the NK gene complex.

Ziegler SF, Levin SD, Johnson L, Copeland NG, Gilbert DJ, Jenkins NA, Baker E, Sutherland GR, Feldhaus AL, Ramsdell F
Journal of immunology (Baltimore, Md. : 1950) 1994 Feb 1;152(3):1228-36

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Very early (VEA) and very late (VLA) activation antigens have distinct functions in T lymphocyte activation.

Yokoyama WM, Maxfield SR, Shevach EM
Immunological reviews 1989 Jun;109:153-76

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Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation.

Yokoyama WM, Koning F, Kehn PJ, Pereira GM, Stingl G, Coligan JE, Shevach EM
Journal of immunology (Baltimore, Md. : 1950) 1988 Jul 15;141(2):369-76

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Fresh BALB/c spleen cells were activated by incubation for 20 hours with PMA + ionomycin, then stained with either hamster IgG-FITC (as an isotype control) or Cd69 monoclonal antibody, clone H1.2F3 (FITC) (Cat # MAB5742). Large, activated cells were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).

Validation comment

Flow Cytometry