PA5-27233

antibody from Invitrogen Antibodies

Targeting: CD82 IA4, KAI1, R2, ST6, TSPAN27

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27233

Provider

Invitrogen Antibodies

Product name

CD82 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: K562.

Concentration

1 mg/mL

References

Submitted references

Muscle cells of sporadic amyotrophic lateral sclerosis patients secrete neurotoxic vesicles.

Le Gall L, Duddy WJ, Martinat C, Mariot V, Connolly O, Milla V, Anakor E, Ouandaogo ZG, Millecamps S, Lainé J, Vijayakumar UG, Knoblach S, Raoul C, Lucas O, Loeffler JP, Bede P, Behin A, Blasco H, Bruneteau G, Del Mar Amador M, Devos D, Henriques A, Hesters A, Lacomblez L, Laforet P, Langlet T, Leblanc P, Le Forestier N, Maisonobe T, Meininger V, Robelin L, Salachas F, Stojkovic T, Querin G, Dumonceaux J, Butler Browne G, González De Aguilar JL, Duguez S, Pradat PF
Journal of cachexia, sarcopenia and muscle 2022 Apr;13(2):1385-1402

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CD82 Is a Marker for Prospective Isolation of Human Muscle Satellite Cells and Is Linked to Muscular Dystrophies.

Alexander MS, Rozkalne A, Colletta A, Spinazzola JM, Johnson S, Rahimov F, Meng H, Lawlor MW, Estrella E, Kunkel LM, Gussoni E
Cell stem cell 2016 Dec 1;19(6):800-807

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CD82 Polyclonal Antibody (Product # PA5-27233). Sample (30 µg of whole cell lysate). Lane A: K562. 12% SDS PAGE. CD82 Polyclonal Antibody (Product # PA5-27233) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of CD82 was achieved by transfecting HeLa with CD82 specific siRNAs (Silencer® select Product # s7672; s223957). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the CD82 knockdown cells (lane 2) and non-targeted siRNA transfected cells (lane 1). The blots were probed with CD82 Polyclonal Antibody (Product # PA5-27233, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to CD82.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of Raji (Lane 1), HeLa (Lane 2), U-87 MG (Lane 3), SK-OV-3 (Lane 4), LNCaP (Lane 5) and PC-3 (Lane 6). The blot was probed with Anti-CD82 Polyclonal Antibody (Product # PA5-27233, 1:1000 dilution) and detected by chemiluminescence using Goat anti Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 27 kDa band corresponding to CD82 was observed across cell lines tested.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human breast cancer, using CD82 (Product # PA5-27233) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL