13801-1-AP

antibody from Invitrogen Antibodies

Targeting: SERPINE1 PAI, PAI1, PLANH1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

13801-1-AP

Provider

Invitrogen Antibodies

Product name

PAI-1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MQMSPALTC LVLGLALVFG EGSAVHHPPS YVAHLASDFG VRVFQQVAQA SKDRNVVFSP YGVASVLAML QLTTGGETQQ QIQAAMGFKI DDKGMAPALR HLYKELMGPW NKDEISTTDA IFVQRDLKLV QGFMPHFFRL FRSTVKQVDF SEVERARFII NDWVKTHTKG MISNLLGKGA VDQLTRLVLV NALYFNGQWK TPFPDSSTHR RLFHKSDGST VSVPMMAQTN KFNYTEFTTP DGHYYDILEL PYHGDTLSMF IAAPYEKEVP LSALTNILSA QLISHWKGNM TRLPRLLVLP KFSLETEVDL RKPLENLGMT DMFRQFQADF TSLSDQEPLH VAQALQKVKI EVNESGTVAS SSTAVIVSAR MAPEEIIMDR PFLFVVRHNP TGTVLFMGQV MEP (1-402 aa encoded by BC010860)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 13801-1-AP (PAI-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-12 cells were subjected to SDS PAGE followed by western blot with 13801-1-AP ( PAI-1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using PAI-1 antibody (Product # 13801-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 13801-1-AP (PAI-1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 13801-1-AP (PAI-1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 13801-1-AP ( PAI-1 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 13801-1-AP ( PAI-1 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PAI-1 (IP: 13801-1-AP, 3 µg; Detection: 13801-1-AP 1:300) with SH-SY5Y cells lysate 2000 µg.