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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- PAB11269 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11269, RRID:AB_1714667
- Product name
- SPRY4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of SPRY4.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Genomic structure and promoter characterization of the human Sprouty4 gene, a novel regulator of lung morphogenesis.
Mammalian Sprouty4 suppresses Ras-independent ERK activation by binding to Raf1.
Human sprouty 4, a new ras antagonist on 5q31, interacts with the dual specificity kinase TESK1.
Ding W, Bellusci S, Shi W, Warburton D
American journal of physiology. Lung cellular and molecular physiology 2004 Jul;287(1):L52-9
American journal of physiology. Lung cellular and molecular physiology 2004 Jul;287(1):L52-9
Mammalian Sprouty4 suppresses Ras-independent ERK activation by binding to Raf1.
Sasaki A, Taketomi T, Kato R, Saeki K, Nonami A, Sasaki M, Kuriyama M, Saito N, Shibuya M, Yoshimura A
Nature cell biology 2003 May;5(5):427-32
Nature cell biology 2003 May;5(5):427-32
Human sprouty 4, a new ras antagonist on 5q31, interacts with the dual specificity kinase TESK1.
Leeksma OC, Van Achterberg TA, Tsumura Y, Toshima J, Eldering E, Kroes WG, Mellink C, Spaargaren M, Mizuno K, Pannekoek H, de Vries CJ
European journal of biochemistry / FEBS 2002 May;269(10):2546-56
European journal of biochemistry / FEBS 2002 May;269(10):2546-56
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using SPRY4 polyclonal antibody (Cat # PAB11269) shows detection of a doublet band ~35 kDa corresponding to human SPRY4.Approximately 30 ug of HeLa (lane 1), SW-13 (lane 2) and C2C12 (Lane 3) whole cell lysates were separated by 10% SDS-PAGE.The primary antibody diluted to 1:100.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of SPRY4 polyclonal antibody (Cat # PAB11269) shows strong cytoplasmic and membranous staining of tumor cells in human liver tissue.Tissue was formalin-fixed and paraffin embedded.Brown color indicates presence of protein, blue color shows cell nuclei.Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of SPRY4 polyclonal antibody (Cat # PAB11269) was used at a 2.5 ug/mL to detect cytoplasmic signal with moderate to strong staining and low background in a variety of tissues including heart, prostate, intestine, kidney and pancreas.Strong specific staining was noted at particular cell types in some tissues (i.e. Sertoli and Leydig cells).The antibody shows very good signal to background at 2.5 ug/mL.This image shows SPRY4 staining of human heart tissue.Tissue was formalin-fixed and paraffin embedded.Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
