AP09263PU-N

antibody from Acris Antibodies GmbH

Targeting: SPRY4

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09263PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09263PU-N, RRID:AB_2035980

Product name

anti Sprouty homolog 4 / SPRY4 (306-322)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids 306-322 of Human Sprouty-4 protein

Reactivity

Human, Mouse, Rat, Bovine, Canine

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

0.93 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using anti-Sprouty-4 antibody shows detection of a doublet band ~35 kDa corresponding to human Sprouty-4. Approximately 30 μg of HeLa (lane 1), SW13 (lane 2) and C2C12 (Lane 3) whole cell lysates were separated by 10% SDS-PAGE and transferred ontonitrocellulose. After blocking with 5% non-fat dry milk in TBST the membrane was probed with the primary antibody diluted to 1:100 overnight at 4°C followed by washes and reaction with a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG [H&L] for 1 h at room temperature. Signal was processed by ECL (Pierce). Other detection systems will yield similar results.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry. Anti-Sprouty-4 antibody was used at a 2.5 μg/ml to detect cytoplasmic signal with moderate to strong staining and low background in a variety of tissues including heart, prostate, intestine, kidney and pancreas. Strong specific staining was noted at particular cell types in some tissues (i.e. Sertoli and Leydig cells). The antibody shows very good signal to background at 2.5 µg/ml. This image shows Sprouty-4 staining of human heart tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry. Anti-Sprouty-4 antibody shows strong cytoplasmic and membranous staining of tumor cells in human liver tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.