H00007020-M01

antibody from Abnova Corporation

Targeting: TFAP2A AP-2, AP2TF, TFAP2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007020-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007020-M01, RRID:AB_490092

Product name

TFAP2A monoclonal antibody (M01), clone 2G5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant TFAP2A.

Antigen sequence

SGLLHTHRGLPHQLSGLDPRRDYRRHEDLLHGPHA
LSSGLGDLSIHSLPHAIEEVPHVEDPGINIPDQTV
IKKGPVSLSKSNSNAVSAIPINKDNLFGGVVNPNE
VF


Isotype

IgG

Antibody clone number

2G5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of TFAP2A expression in transfected 293T cell line by TFAP2A monoclonal antibody (M01), clone 2G5.Lane 1: TFAP2A transfected lysate(48.1 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged TFAP2A is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to TFAP2A on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol