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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- ELISA [1]
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- Product number
- H00007391-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00007391-M01, RRID:AB_549054
- Product name
- Mouse Monoclonal USF1 Antibody
- Antibody type
- Monoclonal
- Description
- Ascites. USF1 - upstream transcription factor 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references A role of DNA-PK for the metabolic gene regulation in response to insulin.
Wong RH, Chang I, Hudak CS, Hyun S, Kwan HY, Sul HS
Cell 2009 Mar 20;136(6):1056-72
Cell 2009 Mar 20;136(6):1056-72
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USF1 Antibody (3F6) [H00007391-M01] - Analysis of USF1 over-expressed 293 cell line, cotransfected with USF1 Validated Chimera RNAi ( Cat # H00007391-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with USF1 monoclonal antibody (M01), clone 3F6 (Cat # H00007391-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USF1 Antibody (3F6) [H00007391-M01] - Analysis of USF1 expression in transfected 293T cell line by USF1 monoclonal antibody (M01), clone 3F6.Lane 1: USF1 transfected lysate(33.5 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USF1 Antibody (3F6) [H00007391-M01] - USF1 monoclonal antibody (M01), clone 3F6 Analysis of USF1 expression in K-562.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: USF1 Antibody (3F6) [H00007391-M01] - Detection limit for recombinant GST tagged USF1 is approximately 0.03ng/ml as a capture antibody.
