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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MAB2864 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Rat SOX10 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and rat SOX10. Epitope-mapping experiments indicated that clone 20B7 recognizes a determinant in the first 65 amino acids, which contains sequences unique to SOX10 (1).
- Reactivity
- Human, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 20B7
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Identification and functional analysis of SOX10 phosphorylation sites in melanoma.
Schwann cell-like differentiated adipose stem cells promote neurite outgrowth via secreted exosomes and RNA transfer.
Analysis of induced pluripotent stem cells carrying 22q11.2 deletion.
Oligodendroglial maturation is dependent on intracellular protein shuttling.
Perivascular-derived stem cells with neural crest characteristics are involved in tendon repair.
Modulation of lipopolysaccharide-induced neuronal response by activation of the enteric nervous system.
HDAC1 and HDAC2 control the specification of neural crest cells into peripheral glia.
HDAC1 and HDAC2 control the transcriptional program of myelination and the survival of Schwann cells.
A highly enriched niche of precursor cells with neuronal and glial potential within the hair follicle dermal papilla of adult skin.
Cronin JC, Loftus SK, Baxter LL, Swatkoski S, Gucek M, Pavan WJ
PloS one 2018;13(1):e0190834
PloS one 2018;13(1):e0190834
Schwann cell-like differentiated adipose stem cells promote neurite outgrowth via secreted exosomes and RNA transfer.
Ching RC, Wiberg M, Kingham PJ
Stem cell research & therapy 2018 Oct 11;9(1):266
Stem cell research & therapy 2018 Oct 11;9(1):266
Analysis of induced pluripotent stem cells carrying 22q11.2 deletion.
Toyoshima M, Akamatsu W, Okada Y, Ohnishi T, Balan S, Hisano Y, Iwayama Y, Toyota T, Matsumoto T, Itasaka N, Sugiyama S, Tanaka M, Yano M, Dean B, Okano H, Yoshikawa T
Translational psychiatry 2016 Nov 1;6(11):e934
Translational psychiatry 2016 Nov 1;6(11):e934
Oligodendroglial maturation is dependent on intracellular protein shuttling.
Göttle P, Sabo JK, Heinen A, Venables G, Torres K, Tzekova N, Parras CM, Kremer D, Hartung HP, Cate HS, Küry P
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Jan 21;35(3):906-19
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Jan 21;35(3):906-19
Perivascular-derived stem cells with neural crest characteristics are involved in tendon repair.
Xu W, Sun Y, Zhang J, Xu K, Pan L, He L, Song Y, Njunge L, Xu Z, Chiang MY, Sung KL, Chuong CM, Yang L
Stem cells and development 2015 Apr 1;24(7):857-68
Stem cells and development 2015 Apr 1;24(7):857-68
Modulation of lipopolysaccharide-induced neuronal response by activation of the enteric nervous system.
Coquenlorge S, Duchalais E, Chevalier J, Cossais F, Rolli-Derkinderen M, Neunlist M
Journal of neuroinflammation 2014 Dec 12;11:202
Journal of neuroinflammation 2014 Dec 12;11:202
HDAC1 and HDAC2 control the specification of neural crest cells into peripheral glia.
Jacob C, Lötscher P, Engler S, Baggiolini A, Varum Tavares S, Brügger V, John N, Büchmann-Møller S, Snider PL, Conway SJ, Yamaguchi T, Matthias P, Sommer L, Mantei N, Suter U
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Apr 23;34(17):6112-22
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Apr 23;34(17):6112-22
HDAC1 and HDAC2 control the transcriptional program of myelination and the survival of Schwann cells.
Jacob C, Christen CN, Pereira JA, Somandin C, Baggiolini A, Lötscher P, Ozçelik M, Tricaud N, Meijer D, Yamaguchi T, Matthias P, Suter U
Nature neuroscience 2011 Apr;14(4):429-36
Nature neuroscience 2011 Apr;14(4):429-36
A highly enriched niche of precursor cells with neuronal and glial potential within the hair follicle dermal papilla of adult skin.
Hunt DP, Morris PN, Sterling J, Anderson JA, Joannides A, Jahoda C, Compston A, Chandran S
Stem cells (Dayton, Ohio) 2008 Jan;26(1):163-72
Stem cells (Dayton, Ohio) 2008 Jan;26(1):163-72
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- SOX10 in BG01V Human Embryonic Stem Cells. SOX10 was detected in immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells using Mouse Anti-Human/Rat SOX10 Monoclonal Antibody (Catalog # MAB2864) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- SOX10 in Human Melanoma Tissue. SOX10 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Mouse Anti-Human/Rat SOX10 Monoclonal Antibody (Catalog # MAB2864) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
