BS4720

antibody from Bioworld Technology, Inc

Targeting: AKT2 PKBβ

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

BS4720

Provider

Bioworld Technology, Inc

Proper citation

Bioworld Technology Cat#BS4720, RRID:AB_1663104

Product name

AKT2 (phospho S474) polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic phosphopeptide derived from human AKT2 around the phosphorylation site of Serine 474.

Description

AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100ul

Concentration

1 mg/ml

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Bioworld Technology, Inc (provider)

Main image

Experimental details

Western blot (WB) analysis of p-AKT2 (S474) pAb at 1:500 dilutionLane1:Hela cell lysate treated with TNFaLane2:sp2/0 cell lysate treated with TNFaLane3:PC12 cell lysate treated with H2O2