H00000208-M06

antibody from Abnova Corporation

Targeting: AKT2 PKBβ

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000208-M06

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000208-M06, RRID:AB_10754700

Product name

AKT2 monoclonal antibody (M06), clone X1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant AKT2.

Antigen sequence

MRAIQMVANSLKQRAPGEDPMDYKCGSPSDSSTTE
EMEVAVSKARAKVTMNDFDYLKLLGKGTFGKVILV
REKATGRYYAMKILRKEVII


Isotype

IgG

Antibody clone number

X1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

AKT2 monoclonal antibody (M06), clone X1. Western Blot analysis of AKT2 expression in Raw 264.7(Cat # L024V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged AKT2 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to AKT2 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to AKT2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol