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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- ARG10552 - Provider product page
- Provider
- Arigo
- Product name
- anti-Bax antibody [G26-R]
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide around the N-terminus of Human bax
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- G26-R
- Vial size
- 50 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 200 µg of A) Mouse brain extract, B) Rat brain extract, C) 293 cells, and GST-fusion recombinant Human Bax protein at following amount: D) 100 ng, E) 200 ng, and F) 500 ng. All lanes were stained with ARG10552 anti-Bax antibody [G26-R].
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunocytochemistry: HEK293 cells stained with ARG10552 anti-Bax antibody [G26-R] at 1:100 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunoprecipitation: Bax immunoprecipitated from HEK 293 cells, visualized with ARG10552 anti-Bax antibody [G26-R] in WB at 1:2000 dilution. 1) Marker, 2) 200 ng of recombinant GST tagged BAX (positive control), 3) Immunoprecipitation with 200 ng of recombinant GST tagged BAX (positive control), 4) Immunoprecipitation without primary antibody (negative control), and 5) Immunoprecipitated BAX (~20 kDa) from HEK 293 cells (500 µg of crude protein extract).
