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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-31913 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFYB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HeLa nuclear extract, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2.
- Concentration
- 0.42 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFYB using 20 µg of HeLa nucleus lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a NFYB polyclonal antibody (Product # PA5-31913) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NFYB was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFYB Polyclonal Antibody (Product # PA5-31913) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NFYB Polyclonal Antibody (Product # PA5-31913). HeLa whole cell and nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB Polyclonal Antibody (Product # PA5-31913) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NFYB was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFYB Polyclonal Antibody (Product # PA5-31913) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NFYB was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFYB Polyclonal Antibody (Product # PA5-31913) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NFYB was performed by separating 30 µg of HeLa whole cell and nuclear extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a NFYB Polyclonal Antibody (Product # PA5-31913) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NFYB Polyclonal Antibody (Product # PA5-31913). HeLa whole cell and nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB Polyclonal Antibody (Product # PA5-31913) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NFYB was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: NFYB Polyclonal Antibody (Product # PA5-31913) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NFYB was performed in paraffin-embedded mouse intestine tissue using NFYB Polyclonal Antibody (Product # PA5-31913) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NFYB was performed in paraffin-embedded rat stomach tissue using NFYB Polyclonal Antibody (Product # PA5-31913) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or NFYB Polyclonal Antibody (Product # PA5-31913). The precipitated DNA was detected by PCR with primer set targeting to aurora kinase A.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NFYB Polyclonal Antibody immunoprecipitates NFYB protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 40 µg whole cell lysate/extract of NFYB protein expressing 293T cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of NFYB protein by 2.5 µg of NFYB Polyclonal Antibody (Product # PA5-31913) 10% SDS-PAGE The immunoprecipitated NFYB protein was detected by NFYB Polyclonal Antibody (Product # PA5-31913) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.
