- PA5-27244 - Invitrogen Antibodies CEBPB antibody

Submitted references Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation.

Xian H, Liu Y, Rundberg Nilsson A, Gatchalian R, Crother TR, Tourtellotte WG, Zhang Y, Aleman-Muench GR, Lewis G, Chen W, Kang S, Luevanos M, Trudler D, Lipton SA, Soroosh P, Teijaro J, de la Torre JC, Arditi M, Karin M, Sanchez-Lopez E
Immunity 2021 Jul 13;54(7):1463-1477.e11

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of CEBP beta using 30 µg of A431 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a CEBP beta polyclonal antibody (Product # PA5-27244) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of C/EBP beta was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a C/EBP beta Polyclonal Antibody (Product # PA5-27244) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of CEBPB protein from HeLa nuclear extracts using 5 µg of C/EBP beta polyclonal antibody (Product # PA5-27244). Western blot analysis was performed using C/EBP beta antibody diluted at 1:500. Anti-Rabbit IgG was used as a secondary reagent.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-C/EBP beta Polyclonal Antibody (Product # PA5-27244) and a band at ~36 kDa corresponding to C/EBP beta was observed across cell lines tested. Modified whole cell extracts (30 µg lysate) of A-431 (Lane 1), SK-OV-3 (Lane 2) and HeLa (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunocytochemistry-Immunofluorescence analysis of C/EBP beta was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: C/EBP beta Polyclonal Antibody (Product # PA5 27244) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of C/EBP beta at cytoplasm and nucleus in HeLa cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C/EBP beta stained by C/EBP beta polyclonal antibody (Product # PA5-27244) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using C/EBP beta (Product # PA5-27244) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Cross-linked ChIP was performed with Raji chromatin extract and 5 µg of either control rabbit IgG or C/EBP beta Polyclonal Antibody (Product # PA5-27244). The precipitated DNA was detected by PCR with primer set targeting to CR2pD.

PA5-27244

Antibody data