Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 10972-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CTBP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MGSSHLLNK GLPLGVRPPI MNGPLHPRPL VALLDGRDCT VEMPILKDVA TVAFCDAQST QEIHEKVLNE AVGALMYHTI TLTREDLEKF KALRIIVRIG SGFDNIDIKS AGDLGIAVCN VPAASVEETA DSTLCHILNL YRRATWLHQA LREGTRVQSV EQIREVASGA ARIRGETLGI IGLGRVGQAV ALRAKAFGFN VLFYDPYLSD GVERALGLQR VSTLQDLLFH SDCVTLHCGL NEHNHHLIND FTVKQMRQGA FLVNTARGGL VDEKALAQAL KEGRIRGAAL DVHESEPFSF SQGPLKDAPN LICTPHAAWY SEQASIEMRE EAAREIRRAI TGRIPDSLKN CVNKDHLTAA THWASMDPAV VHPELNGAAY RYPPGVVGVA PTGIPAAVEG IVPSAMSLSH GLPPVAHPPH APSPGQTVKP EADRDHASDQ L (1-440 aa encoded by BC011655)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.17 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HL-60 cells were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTBP1 antibody (Product # 10972-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 10972-1-AP (CTBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 10972-1-AP (CTBP1 antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CTBP1 (IP:10972-1-AP, 3ug; Detection:10972-1-AP 1:200) with mouse brain tissue lysate 4000ug.