AP8823B

antibody from Abgent

Targeting: EWSR1 EWS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP8823B

Provider

Abgent

Proper citation

Abgent Cat#AP8823b, RRID:AB_10552778

Product name

EWSR1 Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.4 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of EWSR1 Antibody (C-term) (Cat. #AP8823b) in A2058 cell line lysates (35ug/lane). EWSR1 (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Fluorescent confocal image of U251 cell stained with EWSR1 Antibody (C-term)(Cat#AP8823b).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EWSR1 primary antibody (1:25, 1 h at 37!). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37!).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37!). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).EWSR1 immunoreactivity is localized to Nucleus significantly."

Primary Ab dilution

1:10~50

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

EWSR1 Antibody (C-term) (Cat. #AP8823b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50