M00589

antibody from Boster Biological Technology

Targeting: EWSR1 EWS

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

M00589

Provider

Boster Biological Technology

Product name

Anti-EWSR1 Antibody Picoband™ (monoclonal, 4B4)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for EWSR1 detection. Tested with WB, IHC-P in Human;Mouse;Monkey.

Reactivity

Human, Mouse, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

4B4

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of EWSR1 using anti- EWSR1 antibody (M00589). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: K562 whole cell lysates, Lane 2: Jurkat whole cell lysates, Lane 3: A549 whole cell lysates, Lane 4: MCF-7 whole cell lysates, Lane 5: COLO-320 whole cell lysates, Lane 6: SW620 whole cell lysates, Lane 7: A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- EWSR1 antigen affinity purified monoclonal antibody (Catalog # M00589) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for EWSR1 at approximately 95KD. The expected band size for EWSR1 is at 95KD.

Additional image