Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-52468 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP2-52468, RRID:AB_2782964
- Product name
- Mouse Monoclonal FGFR3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The Neonatal and Adult Human Testis Defined at the Single-Cell Level.
Sohni A, Tan K, Song HW, Burow D, de Rooij DG, Laurent L, Hsieh TC, Rabah R, Hammoud SS, Vicini E, Wilkinson MF
Cell reports 2019 Feb 5;26(6):1501-1517.e4
Cell reports 2019 Feb 5;26(6):1501-1517.e4
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FGFR3 Antibody (2H10B4) [NBP2-52468] - Analysis using FGFR3 mAb against HEK293 (1) and FGFR3 (AA: 529-694)-hIgGFc transfected HEK293 (2) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FGFR3 Antibody (2H10B4) [NBP2-52468] - Analysis using FGFR3 mAb against human FGFR3 (AA: 529-694) recombinant protein. (Expected MW is 44.1 kDa)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: FGFR3 Antibody (2H10B4) [NBP2-52468] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FGFR3 Antibody (2H10B4) [NBP2-52468] - Analysis of endometrial cancer tissues using FGFR3 mouse mAb with DAB staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: FGFR3 Antibody (2H10B4) [NBP2-52468] - Analysis of ovarian cancer tissues using FGFR3 mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: FGFR3 Antibody (2H10B4) [NBP2-52468] - Analysis of A549 cells using FGFR3 mouse mAb (green) and negative control (red).