GTX21440

antibody from GeneTex

Targeting: HDAC6 FLJ16239, HD6, JM21, KIAA0901, PPP1R90

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX21440

Provider

GeneTex

Proper citation

GeneTex Cat#GTX21440, RRID:AB_371576

Product name

HDAC6 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Sam68 Allows Selective Targeting of Human Cancer Stem Cells.

Benoit YD, Mitchell RR, Risueño RM, Orlando L, Tanasijevic B, Boyd AL, Aslostovar L, Salci KR, Shapovalova Z, Russell J, Eguchi M, Golubeva D, Graham M, Xenocostas A, Trus MR, Foley R, Leber B, Collins TJ, Bhatia M
Cell chemical biology 2017 Jul 20;24(7):833-844.e9

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sonicated Chromatin prepared from untreated (UI) or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with GTX21440 to HDAC6 and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are nomalized over inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 10 ul of GTX21440 and 2x10*6 cells were used in each ChIP experiment

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of HDAC6 using GTX21440 in Jurkat cell lysate