TA800471

antibody from OriGene

Targeting: MAGEB1 CT3.1, DAM10, MAGE-Xp, MAGEL1, MGC9322

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA800471

Provider

OriGene

Proper citation

OriGene Cat#TA800471, RRID:AB_2625474

Product name

MAGEB1 mouse monoclonal antibody, clone OTI4E12 (formerly 4E12)

Antibody type

Monoclonal

Description

MAGEB1 mouse monoclonal antibody, clone OTI4E12 (formerly 4E12)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

MAGEB1

Isotype

IgG

Antibody clone number

OTI4E12

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAGEB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAGEB1 (RC224906, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAGEB1.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MAGEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800471)

Validation comment

IHC