10614-1-AP

antibody from Invitrogen Antibodies

Targeting: PKNOX1 PREP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

10614-1-AP

Provider

Invitrogen Antibodies

Product name

PKNOX1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: NSQLQLQLN QDLSILHQDD GSSKNKRGVL PKHATNVMRS WLFQHIGHPY PTEDEKKQIA AQTNLTLLQV NNWFINARRR ILQPMLDSSC SETPKTKKKT AQNRPVQRFW PDSIASGVAQ PPPSELTMSE GAVVTITTPV NMNVDSLQSL SSDGATLAVQ QVMMAGQSED ESVDSTEEDA GALAPAHISG LVLENSDSLQ (238-436 aa encoded by BC007746)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 10614-1-AP (PKNOX1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COLO 320 cells were subjected to SDS PAGE followed by western blot with 10614-1-AP (PKNOX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10614-1-AP ( PKNOX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 10614-1-AP (PKNOX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 10614-1-AP (PKNOX1 antibody) at dilution of 1:100 (under 25x lens).