11102-1-AP

antibody from Invitrogen Antibodies

Targeting: RANGAP1 Fug1, KIAA1835, SD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11102-1-AP

Provider

Invitrogen Antibodies

Product name

RANGAP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LAQAFAVNP LLRVINLNDN TFTEKGAVAM AETLKTLRQV EVINFGDCLV RSKGAVAIAD AIRGGLPKLK ELNLSFCEIK RDAALAVAEA MADKAELEKL DLNGNTLGEE GCEQLQEVLE GFNMAKVLAS LSDDEDEEEE EEGEEEEEEA EEEEEEDEEE EEEEEEEEEE EPQQRGQGEK SATPSRKILD PNTGEPAPVL SSPPPADVST FLAFPSPEKL LRLGPKSSVL IAQQTDTSDP EKVVSAFLKV SSVFKDEATV RMAVQDAVDA LMQKAFNSSS FNSNTFLTRL LVHMGLLKSE DKVKAIANLY GPLMALNHMV QQDYFPKALA PLLLAFVTKP NSALESCSFA RHSLLQTLYK V (228-587 aa encoded by BC014044)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11102-1-AP ( RANGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 11102-1-AP (RANGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using RANGAP1 antibody 11102-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IGG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11102-1-AP (RANGAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 11102-1-AP ( RANGAP1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 11102-1-AP ( RANGAP1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were stained with 0.2ug RANGAP1 antibody (11102-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RANGAP1 (IP:11102-1-AP, 4ug; Detection:11102-1-AP 1:300) with HEK-293 cells lysate 3600ug.