HPA001085

antibody from Atlas Antibodies

Targeting: SASH3 753P9, CXorf9, HACS2, SH3D6C, SLY

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA001085

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA001085, RRID:AB_2184289

Product name

Anti-SASH3

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

PKTLHELLERIGLEEHTSTLLLNGYQTLEDFKELR
ETHLNELNIMDPQHRAKLLTAAELLLDYDTGSEEA
EEGAESSQEPVAHTVSEPKVDIPRDSGCFEGSESG
RDDAELAGTEEQLQGLSL


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and SASH3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412828).

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human tonsil and skeletal muscle tissues using Anti-SASH3 antibody. Corresponding SASH3 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows low expression as expected.

Sample type

HUMAN