NB110-96869
antibody from Novus Biologicals
Targeting: CAMK2A
CAMKA, CaMKIINalpha, CaMKIIα, KIAA0968
Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
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Validation data
Reference
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- Product number
- NB110-96869 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-96869, RRID:AB_1260569
- Product name
- Mouse Monoclonal CaMKII alpha/beta Antibody
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Protein G purified. This antibody is specific for a and B subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in B).
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references MerTK signaling in macrophages promotes the synthesis of inflammation resolution mediators by suppressing CaMKII activity.
Thioesterase-mediated control of cellular calcium homeostasis enables hepatic ER stress.
Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis.
Calmodulin-dependent protein kinase II/cAMP response element-binding protein/Wnt/β-catenin signaling cascade regulates angiotensin II-induced podocyte injury and albuminuria.
Cai B, Kasikara C, Doran AC, Ramakrishnan R, Birge RB, Tabas I
Science signaling 2018 Sep 25;11(549)
Science signaling 2018 Sep 25;11(549)
Thioesterase-mediated control of cellular calcium homeostasis enables hepatic ER stress.
Ersoy BA, Maner-Smith KM, Li Y, Alpertunga I, Cohen DE
The Journal of clinical investigation 2018 Jan 2;128(1):141-156
The Journal of clinical investigation 2018 Jan 2;128(1):141-156
Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis.
Conlon DM, Thomas T, Fedotova T, Hernandez-Ono A, Di Paolo G, Chan RB, Ruggles K, Gibeley S, Liu J, Ginsberg HN
The Journal of clinical investigation 2016 Oct 3;126(10):3852-3867
The Journal of clinical investigation 2016 Oct 3;126(10):3852-3867
Calmodulin-dependent protein kinase II/cAMP response element-binding protein/Wnt/β-catenin signaling cascade regulates angiotensin II-induced podocyte injury and albuminuria.
Jiang L, Xu L, Song Y, Li J, Mao J, Zhao AZ, He W, Yang J, Dai C
The Journal of biological chemistry 2013 Aug 9;288(32):23368-79
The Journal of biological chemistry 2013 Aug 9;288(32):23368-79
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - analysis of CaMKII and NFAT phosphorylation in ventricles of 14 day old mice over-expressing CaMK.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Bouin's fixed paraffin-embedded backskin sections of transgenic mice- muscle, and hair follicle stiaing. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1 . Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.