10918-1-AP

antibody from Invitrogen Antibodies

Targeting: CDC27 ANAPC3, APC3, D0S1430E, D17S978E, NUC2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10918-1-AP

Provider

Invitrogen Antibodies

Product name

CDC27/APC3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: ALCSYNCKE AINILSHLPS HHYNTGWVLC QIGRAYFELS EYMQAERIFS EVRRIENYRV EGMEIYSTTL WHLQKDVALS VLSKDLTDMD KNSPEAWCAA GNCFSLQREH DIAIKFFQRA IQVDPNYAYA YTLLGHEFVL TEELDKALAC FRNAIRVNPR HYNAWYGLGM IYYKQEKFSL AEMHFQKALD INPQSSVLLC HIGVVQHALK KSEKALDTLN KAIVIDPKNP LCKFHRASVL FANEKYKSAL QELEELKQIV PKESLVYFLI GKVYKKLGQT HLALMNFSWA MDLDPKGANN QIKEAIDKRY LPDDEEPITQ EEQIMGTDES QESSMTDADD TQLHAAESDE F (481-830 aa encoded by BC011656)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.39 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 10918-1-AP (CDC27; APC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 10918-1-AP (CDC27; APC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SH-SY5Y cells, using CDC27 antibody 10918-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IGG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer using 10918-1-AP (CDC27; APC3 antibody) at dilution of 1:50 (under 10x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 K-562 cells were intracellularly stained with 0.2 µg Anti-Human CDC27; APC3 (Product # 10918-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 90% MeOH .

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-CDC27; APC3 (IP:10918-1-AP, 3ug; Detection:10918-1-AP 1:600) with K-562 cells lysate 11000ug.