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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-8401 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-8401, RRID:AB_627233
- Product name
- Anti-CDC42
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Mouse
Submitted references Adipose tissue explants and MDCK cells reciprocally regulate their morphogenesis in coculture
The rho-specific guanine nucleotide exchange factor Dbs regulates breast cancer cell migration.
Intersectin 1L guanine nucleotide exchange activity is regulated by adjacent src homology 3 domains that are also involved in endocytosis.
Quantitative proteomic analysis of Myc oncoprotein function.
Kazuma Udo, Shigehisa Aoki, Kazuyoshi Uchihashi, Maki Kawasaki, Aki Matsunobu, Yuji Tokuda, Akifumi Ootani, Shuji Toda, Jiro Uozumi
Kidney International 2010 Mar;78(1):60-68
Kidney International 2010 Mar;78(1):60-68
The rho-specific guanine nucleotide exchange factor Dbs regulates breast cancer cell migration.
Liu Z, Adams HC 3rd, Whitehead IP
The Journal of biological chemistry 2009 Jun 5;284(23):15771-80
The Journal of biological chemistry 2009 Jun 5;284(23):15771-80
Intersectin 1L guanine nucleotide exchange activity is regulated by adjacent src homology 3 domains that are also involved in endocytosis.
Zamanian JL, Kelly RB
Molecular biology of the cell 2003 Apr;14(4):1624-37
Molecular biology of the cell 2003 Apr;14(4):1624-37
Quantitative proteomic analysis of Myc oncoprotein function.
Shiio Y, Donohoe S, Yi EC, Goodlett DR, Aebersold R, Eisenman RN
The EMBO journal 2002 Oct 1;21(19):5088-96
The EMBO journal 2002 Oct 1;21(19):5088-96
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 21
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
