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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PAB0634 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0634, RRID:AB_982490
- Product name
- YES1 (phospho Y527) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y537 of human YES1.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400 µl
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The Src family kinase Yes triggers hyperosmotic activation of the epidermal growth factor receptor and CD95.
The Src family of tyrosine kinases is important for embryonic stem cell self-renewal.
Regulation of the human sperm tyrosine kinase c-yes. Activation by cyclic adenosine 3',5'-monophosphate and inhibition by Ca(2+).
Reinehr R, Becker S, Höngen A, Haüssinger D
The Journal of biological chemistry 2004 Jun 4;279(23):23977-87
The Journal of biological chemistry 2004 Jun 4;279(23):23977-87
The Src family of tyrosine kinases is important for embryonic stem cell self-renewal.
Annerén C, Cowan CA, Melton DA
The Journal of biological chemistry 2004 Jul 23;279(30):31590-8
The Journal of biological chemistry 2004 Jul 23;279(30):31590-8
Regulation of the human sperm tyrosine kinase c-yes. Activation by cyclic adenosine 3',5'-monophosphate and inhibition by Ca(2+).
Leclerc P, Goupil S
Biology of reproduction 2002 Jul;67(1):301-7
Biology of reproduction 2002 Jul;67(1):301-7
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Jurkat cell lysate with YES1 (phospho Y527) polyclonal antibody (Cat # PAB0634).40 ug of lysate loaded, ECL detection; Lane 1, 2.5 ug/ml; Lane 2, 1 ug/ml; Lane 3, 0.62 ug/ml.
- Validation comment
- Western Blot (Cell lysate)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with YES1 (phospho Y527) polyclonal antibody (Cat # PAB0634) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot blot analysis of YES1 (phospho Y527) polyclonal antibody (Cat # PAB0634) on nitrocellulose membrane. 50 ng of phospho-peptide or non phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5 ug per mL.
- Validation comment
- Dot Blot (Peptide)
