H00011069-M01

antibody from Abnova Corporation

Targeting: RAPGEF4 cAMP-GEFII, CGEF2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00011069-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00011069-M01, RRID:AB_464280

Product name

RAPGEF4 monoclonal antibody (M01), clone 1C11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant RAPGEF4.

Antigen sequence

MVAAHAAHSSSSAEWIACLDKRPLERSSEDVDIIF
TRLKEVKAFEKFHPNLLHQICLCGYYENLEKGITL
FRQGDIGTNWYAVLAGSLDVKVSETSSHQDAVTIC
TLGIG


Isotype

IgG

Antibody clone number

1C11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RAPGEF4 expression in transfected 293T cell line by RAPGEF4 monoclonal antibody (M01), clone 1C11.Lane 1: RAPGEF4 transfected lysate (Predicted MW: 115.5 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RAPGEF4 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol