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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- PA5-32394 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-ERCC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is breast carcinoma.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Concentration
- Lot Dependent
- Storage
- 4° C, do not freeze
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of ERCC1 using anti-ERCC1 Polyclonal Antibody (Product # PA5-32394) in Lung SCC Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.
