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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- TA500330 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500330, RRID:AB_2294376
- Product name
- Anti-GAD1 (GAD67) mouse monoclonal antibody, clone OTI5D8 (formerly 5D8)
- Antibody type
- Monoclonal
- Description
- Anti-GAD1 (GAD67) mouse monoclonal antibody, clone OTI5D8 (formerly 5D8)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GAD1
- Isotype
- IgG
- Antibody clone number
- OTI5D8
- Vial size
- 100 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAD1 (RC207226, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAD1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-GAD1 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation of GAD1 by using TrueMab monoclonal anti-GAD1 antibodies (Negative control: IP without adding anti-GAD1 antibody.). For each experiment, 500ul of DDK tagged GAD1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GAD1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500330, Dilution 1:50)
- Validation comment
- IHC
