NBP1-48272

antibody from Novus Biologicals

Targeting: GAD1 GAD

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-48272

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-48272, RRID:AB_10010732

Product name

Mouse Monoclonal GAD1/GAD67 Antibody

Antibody type

Monoclonal

Description

Affinity purified. This antibody is specific for Homo sapiens glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67.

Reactivity

Human, Simian

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

0.46 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Characterization of continuous B-cell epitopes in the N-terminus of glutamate decarboxylase67 using monoclonal antibodies.

Agca S, Houen G, Trier NH
Journal of peptide science : an official publication of the European Peptide Society 2014 Dec;20(12):928-34

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GAD1/GAD67 monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAD1/GAD67 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAD1/GAD67.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GAD1/GAD67 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GAD1/GAD67 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GAD1/GAD67 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GAD1/GAD67 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Human colon tissue using anti-GAD1/GAD67 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Human endometrium tissue using anti-GAD1/GAD67 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Human lung tissue using anti-GAD1/GAD67 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Human Ovary tissue using anti-GAD1/GAD67 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (OTI3G9) [NBP1-48272] - Staining of paraffin-embedded Human pancreas tissue using anti-GAD1/GAD67 mouse monoclonal antibody.