AF1590

antibody from R&D Systems

Targeting: GSK3A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1590

Provider

R&D Systems

Product name

Human/Mouse/Rat Phospho-GSK-3 alpha/beta (S21/S9) Antibody

Antibody type

Polyclonal

Description

Antigen and protein A Affinity-purified. Detects human, mouse, and rat GSK-3 beta when phosphorylated at S9, and human, mouse, and rat GSK-3 alpha when phosphorylated at S21.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

HYS-32-Induced Microtubule Catastrophes in Rat Astrocytes Involves the PI3K-GSK3beta Signaling Pathway.

Chiu CT, Liao CK, Shen CC, Tang TK, Jow GM, Wang HS, Wu JC
PloS one 2015;10(5):e0126217

---

Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response.

Lutay N, Håkansson G, Alaridah N, Hallgren O, Westergren-Thorsson G, Godaly G
PloS one 2014;9(1):e86466

---

Mechanical induction of PGE2 in osteocytes blocks glucocorticoid-induced apoptosis through both the β-catenin and PKA pathways.

Kitase Y, Barragan L, Qing H, Kondoh S, Jiang JX, Johnson ML, Bonewald LF
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2010 Dec;25(12):2657-68

---

Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation.

Tang Z, Arjunan P, Lee C, Li Y, Kumar A, Hou X, Wang B, Wardega P, Zhang F, Dong L, Zhang Y, Zhang SZ, Ding H, Fariss RN, Becker KG, Lennartsson J, Nagai N, Cao Y, Li X
The Journal of experimental medicine 2010 Apr 12;207(4):867-80

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Phospho-GSK-3 alpha (S21) and GSK-3 beta (S9) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes and MCF-7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291-G1) for 15 minutes. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse/Rat Phospho-GSK-3 alpha/beta (S21/S9) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1590), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-GSK-3 alpha (S21) and GSK-3 beta (S9) at approximately 51 and 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Phospho-GSK-3 alpha/beta (S21/S9) in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cells, untreated (open histogram, yellow line), or treated with PI3-kinase inhibitors (dark orange filled histogram), were stained with Human/Mouse/Rat Phospho-GSK-3 alpha/beta (S21/S9) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1590) or control antibody (Catalog # AB-105-C, blue open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.