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LearnADI-SPA-470-F
antibody from Enzo Life Sciences
Targeting: SERPINH1
CBP1, CBP2, colligen, HSP47, SERPINH2
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [24]
- Comments [0]
- Validations
- Immunocytochemistry [3]
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- Product number
- ADI-SPA-470-F - Provider product page
- Provider
- Enzo Life Sciences
- Product name
- HSP47 monoclonal antibody (M16.10A1)
- Antibody type
- Monoclonal
- Antigen
- Purified from natural sources
- Description
- Protein G affinity purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Guinea Pig, Hamster, Porcine, Rabbit, Sheep, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- M16.10A1
- Vial size
- 200 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Mutants of collagen-specific molecular chaperone Hsp47 causing osteogenesis imperfecta are structurally unstable with weak binding affinity to collagen.
The kampo medicine Daikenchuto inhibits peritoneal fibrosis in mice.
Yip1A, a novel host factor for the activation of the IRE1 pathway of the unfolded protein response during Brucella infection.
Molecular Consequences of the SERPINH1/HSP47 Mutation in the Dachshund Natural Model of Osteogenesis Imperfecta.
Bleomycin delivery by osmotic minipump: similarity to human scleroderma interstitial lung disease.
Primary fibroblasts of NDUFS4(-/-) mice display increased ROS levels and aberrant mitochondrial morphology.
Tumor budding, myofibroblast proliferation, and fibrosis in obstructing colon carcinoma: the roles of Hsp47 and basic fibroblast growth factor.
Renoprotective effect of azelnidipine in rats.
Significant synovial pathology in a meniscectomy model of osteoarthritis: modification by intra-articular hyaluronan therapy.
Novel adenoviral gene delivery system targeted against head and neck cancer.
Role of heat shock protein 47, a collagen-binding chaperone, in lacrimal gland pathology in patients with cGVHD.
Col4a1 mutation causes endoplasmic reticulum stress and genetically modifiable ocular dysgenesis.
Specific recognition of the collagen triple helix by chaperone HSP47: minimal structural requirement and spatial molecular orientation.
Specific recognition of the collagen triple helix by chaperone HSP47. II. The HSP47-binding structural motif in collagens and related proteins.
Proteomic analysis of primary cultures of human adipose-derived stem cells: modulation by Adipogenesis.
Immunohistochemical characterization of cells in adult human patellar tendons.
Immunohistochemical expression of angiogenesis-related markers in oral squamous cell carcinomas with multiple metastatic lymph nodes.
Localisation and phenotypical characterisation of collagen-producing cells in TGF-beta 1-induced renal interstitial fibrosis.
Prevalence of HSP47 antigen and autoantibodies to HSP47 in the sera of patients with mixed connective tissue disease.
Sequence-specific recognition of collagen triple helices by the collagen-specific molecular chaperone HSP47.
Functional linkage between the endoplasmic reticulum protein Hsp47 and procollagen expression in human vascular smooth muscle cells.
HSP47 expression by smooth muscle cells is increased during arterial development and lesion formation and is inhibited by fibrillar collagen.
Role of atrophic tubules in development of interstitial fibrosis in microembolism-induced renal failure in rat.
Intracellular retention of procollagen within the endoplasmic reticulum is mediated by prolyl 4-hydroxylase.
Ito S, Nagata K
Biochemical and biophysical research communications 2016 Jan 15;469(3):437-42
Biochemical and biophysical research communications 2016 Jan 15;469(3):437-42
The kampo medicine Daikenchuto inhibits peritoneal fibrosis in mice.
Kitamura M, Nishino T, Obata Y, Oka S, Abe S, Muta K, Ozono Y, Koji T, Kohno S
Biological & pharmaceutical bulletin 2015;38(2):193-200
Biological & pharmaceutical bulletin 2015;38(2):193-200
Yip1A, a novel host factor for the activation of the IRE1 pathway of the unfolded protein response during Brucella infection.
Taguchi Y, Imaoka K, Kataoka M, Uda A, Nakatsu D, Horii-Okazaki S, Kunishige R, Kano F, Murata M
PLoS pathogens 2015 Mar;11(3):e1004747
PLoS pathogens 2015 Mar;11(3):e1004747
Molecular Consequences of the SERPINH1/HSP47 Mutation in the Dachshund Natural Model of Osteogenesis Imperfecta.
Lindert U, Weis MA, Rai J, Seeliger F, Hausser I, Leeb T, Eyre D, Rohrbach M, Giunta C
The Journal of biological chemistry 2015 Jul 17;290(29):17679-89
The Journal of biological chemistry 2015 Jul 17;290(29):17679-89
Bleomycin delivery by osmotic minipump: similarity to human scleroderma interstitial lung disease.
Lee R, Reese C, Bonner M, Tourkina E, Hajdu Z, Riemer EC, Silver RM, Visconti RP, Hoffman S
American journal of physiology. Lung cellular and molecular physiology 2014 Apr 15;306(8):L736-48
American journal of physiology. Lung cellular and molecular physiology 2014 Apr 15;306(8):L736-48
Primary fibroblasts of NDUFS4(-/-) mice display increased ROS levels and aberrant mitochondrial morphology.
Valsecchi F, Grefte S, Roestenberg P, Joosten-Wagenaars J, Smeitink JA, Willems PH, Koopman WJ
Mitochondrion 2013 Sep;13(5):436-43
Mitochondrion 2013 Sep;13(5):436-43
Tumor budding, myofibroblast proliferation, and fibrosis in obstructing colon carcinoma: the roles of Hsp47 and basic fibroblast growth factor.
Xu CJ, Mikami T, Nakamura T, Tsuruta T, Nakada N, Yanagisawa N, Jiang SX, Okayasu I
Pathology, research and practice 2013 Feb 15;209(2):69-74
Pathology, research and practice 2013 Feb 15;209(2):69-74
Renoprotective effect of azelnidipine in rats.
Kurashige T, Abe K, Furusu A, Miyazaki M, Obata Y, Xia Z, Nakazawa M, Nakazawa Y, Funakoshi S, Harada T, Koji T, Kohno S
Biological & pharmaceutical bulletin 2008 Dec;31(12):2237-44
Biological & pharmaceutical bulletin 2008 Dec;31(12):2237-44
Significant synovial pathology in a meniscectomy model of osteoarthritis: modification by intra-articular hyaluronan therapy.
Smith MM, Cake MA, Ghosh P, Schiavinato A, Read RA, Little CB
Rheumatology (Oxford, England) 2008 Aug;47(8):1172-8
Rheumatology (Oxford, England) 2008 Aug;47(8):1172-8
Novel adenoviral gene delivery system targeted against head and neck cancer.
Li D, Guang W, Abuzeid WM, Roy S, Gao GP, Sauk JJ, O'Malley BW Jr
The Laryngoscope 2008 Apr;118(4):650-8
The Laryngoscope 2008 Apr;118(4):650-8
Role of heat shock protein 47, a collagen-binding chaperone, in lacrimal gland pathology in patients with cGVHD.
Ogawa Y, Razzaque MS, Kameyama K, Hasegawa G, Shimmura S, Kawai M, Okamoto S, Ikeda Y, Tsubota K, Kawakami Y, Kuwana M
Investigative ophthalmology & visual science 2007 Mar;48(3):1079-86
Investigative ophthalmology & visual science 2007 Mar;48(3):1079-86
Col4a1 mutation causes endoplasmic reticulum stress and genetically modifiable ocular dysgenesis.
Gould DB, Marchant JK, Savinova OV, Smith RS, John SW
Human molecular genetics 2007 Apr 1;16(7):798-807
Human molecular genetics 2007 Apr 1;16(7):798-807
Specific recognition of the collagen triple helix by chaperone HSP47: minimal structural requirement and spatial molecular orientation.
Koide T, Asada S, Takahara Y, Nishikawa Y, Nagata K, Kitagawa K
The Journal of biological chemistry 2006 Feb 10;281(6):3432-8
The Journal of biological chemistry 2006 Feb 10;281(6):3432-8
Specific recognition of the collagen triple helix by chaperone HSP47. II. The HSP47-binding structural motif in collagens and related proteins.
Koide T, Nishikawa Y, Asada S, Yamazaki CM, Takahara Y, Homma DL, Otaka A, Ohtani K, Wakamiya N, Nagata K, Kitagawa K
The Journal of biological chemistry 2006 Apr 21;281(16):11177-85
The Journal of biological chemistry 2006 Apr 21;281(16):11177-85
Proteomic analysis of primary cultures of human adipose-derived stem cells: modulation by Adipogenesis.
DeLany JP, Floyd ZE, Zvonic S, Smith A, Gravois A, Reiners E, Wu X, Kilroy G, Lefevre M, Gimble JM
Molecular & cellular proteomics : MCP 2005 Jun;4(6):731-40
Molecular & cellular proteomics : MCP 2005 Jun;4(6):731-40
Immunohistochemical characterization of cells in adult human patellar tendons.
Chuen FS, Chuk CY, Ping WY, Nar WW, Kim HL, Ming CK
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2004 Sep;52(9):1151-7
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2004 Sep;52(9):1151-7
Immunohistochemical expression of angiogenesis-related markers in oral squamous cell carcinomas with multiple metastatic lymph nodes.
Nikitakis NG, Rivera H, Lopes MA, Siavash H, Reynolds MA, Ord RA, Sauk JJ
American journal of clinical pathology 2003 Apr;119(4):574-86
American journal of clinical pathology 2003 Apr;119(4):574-86
Localisation and phenotypical characterisation of collagen-producing cells in TGF-beta 1-induced renal interstitial fibrosis.
Chai Q, Krag S, Chai S, Ledet T, Wogensen L
Histochemistry and cell biology 2003 Apr;119(4):267-80
Histochemistry and cell biology 2003 Apr;119(4):267-80
Prevalence of HSP47 antigen and autoantibodies to HSP47 in the sera of patients with mixed connective tissue disease.
Yokota S, Kubota H, Matsuoka Y, Naitoh M, Hirata D, Minota S, Takahashi H, Fujii N, Nagata K
Biochemical and biophysical research communications 2003 Apr 4;303(2):413-8
Biochemical and biophysical research communications 2003 Apr 4;303(2):413-8
Sequence-specific recognition of collagen triple helices by the collagen-specific molecular chaperone HSP47.
Tasab M, Jenkinson L, Bulleid NJ
The Journal of biological chemistry 2002 Sep 20;277(38):35007-12
The Journal of biological chemistry 2002 Sep 20;277(38):35007-12
Functional linkage between the endoplasmic reticulum protein Hsp47 and procollagen expression in human vascular smooth muscle cells.
Rocnik EF, van der Veer E, Cao H, Hegele RA, Pickering JG
The Journal of biological chemistry 2002 Oct 11;277(41):38571-8
The Journal of biological chemistry 2002 Oct 11;277(41):38571-8
HSP47 expression by smooth muscle cells is increased during arterial development and lesion formation and is inhibited by fibrillar collagen.
Rocnik E, Saward L, Pickering JG
Arteriosclerosis, thrombosis, and vascular biology 2001 Jan;21(1):40-6
Arteriosclerosis, thrombosis, and vascular biology 2001 Jan;21(1):40-6
Role of atrophic tubules in development of interstitial fibrosis in microembolism-induced renal failure in rat.
Suzuki T, Kimura M, Asano M, Fujigaki Y, Hishida A
The American journal of pathology 2001 Jan;158(1):75-85
The American journal of pathology 2001 Jan;158(1):75-85
Intracellular retention of procollagen within the endoplasmic reticulum is mediated by prolyl 4-hydroxylase.
Walmsley AR, Batten MR, Lad U, Bulleid NJ
The Journal of biological chemistry 1999 May 21;274(21):14884-92
The Journal of biological chemistry 1999 May 21;274(21):14884-92
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of HSP47, mAb (M16.10A1) (Prod. no. ADI-SPA-470): Lane 1: Rat recombinant HSP47; Lane 2: Rat-2 (heat shocked); Lane 3: L929 (heat shocked); Lane 4: 3T3 (heat shocked); Lane 5: CHO (heat shocked); Lane 6: HeLa (heat shocked).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human breast cancer tissue immunohistochemically stained using Hsp47 mAb (M16.10A1).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded tissue section of rat carotid artery 14 days after balloon-injury to the artery, stained using Hsp47 (Colligin) mAb (M16.10A1).
