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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-46665 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-46665, RRID:AB_627895
- Product name
- Anti-HELLS
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references LSH and G9a/GLP complex are required for developmentally programmed DNA methylation.
LSH cooperates with DNA methyltransferases to repress transcription.
Myant K, Termanis A, Sundaram AY, Boe T, Li C, Merusi C, Burrage J, de Las Heras JI, Stancheva I
Genome research 2011 Jan;21(1):83-94
Genome research 2011 Jan;21(1):83-94
LSH cooperates with DNA methyltransferases to repress transcription.
Myant K, Stancheva I
Molecular and cellular biology 2008 Jan;28(1):215-26
Molecular and cellular biology 2008 Jan;28(1):215-26
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 35
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human lymph node shows distinct nuclear positivity in reaction center cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
