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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-17785 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-17785, RRID:AB_627921
- Product name
- Anti-MEF2A
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references Proline isomerase Pin1 represses terminal differentiation and myocyte enhancer factor 2C function in skeletal muscle cells.
UTX mediates demethylation of H3K27me3 at muscle-specific genes during myogenesis.
p38 MAPK signaling regulates recruitment of Ash2L-containing methyltransferase complexes to specific genes during differentiation
Transcription enhancer factor 1 binds multiple muscle MEF2 and A/T-rich elements during fast-to-slow skeletal muscle fiber type transitions.
Magli A, Angelelli C, Ganassi M, Baruffaldi F, Matafora V, Battini R, Bachi A, Messina G, Rustighi A, Del Sal G, Ferrari S, Molinari S
The Journal of biological chemistry 2010 Nov 5;285(45):34518-27
The Journal of biological chemistry 2010 Nov 5;285(45):34518-27
UTX mediates demethylation of H3K27me3 at muscle-specific genes during myogenesis.
Seenundun S, Rampalli S, Liu QC, Aziz A, Palii C, Hong S, Blais A, Brand M, Ge K, Dilworth FJ
The EMBO journal 2010 Apr 21;29(8):1401-11
The EMBO journal 2010 Apr 21;29(8):1401-11
p38 MAPK signaling regulates recruitment of Ash2L-containing methyltransferase complexes to specific genes during differentiation
Shravanti Rampalli, LiFang Li, Esther Mak, Kai Ge, Marjorie Brand, Stephen J Tapscott, F Jeffrey Dilworth
Nature Structural & Molecular Biology 2007 Nov;14(12):1150-1156
Nature Structural & Molecular Biology 2007 Nov;14(12):1150-1156
Transcription enhancer factor 1 binds multiple muscle MEF2 and A/T-rich elements during fast-to-slow skeletal muscle fiber type transitions.
Karasseva N, Tsika G, Ji J, Zhang A, Mao X, Tsika R
Molecular and cellular biology 2003 Aug;23(15):5143-64
Molecular and cellular biology 2003 Aug;23(15):5143-64
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 55
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in myocytes.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
