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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Chromatin Immunoprecipitation [1]
- Other assay [2]
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- Product number
- PA5-85554 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SIN3A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution.
- Concentration
- 1.38 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SIN3A in various tissue extracts using SIN3A polyclonal antibody (Product # PA5-85554) using 50 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SIN3A in A) 293T whole cell lysate, B) A431 whole cell lysate, C) HeLa whole cell lysate, D) HepG2 whole cell lysate using SIN3A polyclonal antibody (Product # PA5-85554) at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SIN3A Polyclonal Antibody (Product # PA5-85554). Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SIN3A Polyclonal Antibody (Product # PA5-85554) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SIN3A Polyclonal Antibody (Product # PA5-85554). HeLa whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SIN3A Polyclonal Antibody (Product # PA5-85554) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SIN3A Polyclonal Antibody detects SIN3A protein by western blot analysis. A 293T whole cell lysate/extract. B A431 whole cell lysate/extract. C HeLa whole cell lysate/extract. D HepG2 whole cell lysate/extract.5% SDS-PAGE. SIN3A Polyclonal Antibody (Product # PA5-85554) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of SIN3A in 4% paraformaldehyde-fixed HeLa cells using SIN3A polyclonal antibody (Product # PA5-85554) at a dilution of 1:1000. Sample was then incubated with Hoechst secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SIN3A Polyclonal Antibody detects SIN3A protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SIN3A stained by SIN3A Polyclonal Antibody (Product # PA5-85554) diluted at 1:1,000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SIN3A Polyclonal Antibody detects SIN3A protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. SIN3A Polyclonal Antibody (Product # PA5-85554) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SIN3A Polyclonal Antibody detects SIN3A protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. SIN3A Polyclonal Antibody (Product # PA5-85554) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SIN3A Polyclonal Antibody detects SIN3A protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. SIN3A Polyclonal Antibody (Product # PA5-85554) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with MCF-7 chromatin extract treated with B-estradiol (10 nM for 45 min) and 5 µg of either control rabbit IgG or SIN3A Polyclonal Antibody (Product # PA5-85554). The precipitated DNA was detected by PCR with primer set targeting to GAPDH or MyoD.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of SIN3A in A) 293T whole cell lysate of SIN3A protein expressing 293T cells, B) control of pre-immune rabbit IgG, C) immunoprecipitation of SIN3A protein with SIN3A polyclonal antibody (Product # PA5-85554) using A) 30 µg, B)3 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SIN3A Polyclonal Antibody immunoprecipitates SIN3A protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 µg whole cell lysate/extract of SIN3A protein expressing 293T cells B : Control with 3 µg of pre-immune rabbit IgG C : Immunoprecipitation of SIN3A by 3 µg of SIN3A Polyclonal Antibody (Product # PA5-85554) 5% SDS-PAGE The immunoprecipitated SIN3A protein was detected by SIN3A Polyclonal Antibody (Product # PA5-85554) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.
