sc-5307

antibody from Santa Cruz Biotechnology

Targeting: NRP1 CD304, NRP, VEGF165R

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-5307

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-5307, RRID:AB_2282634

Product name

Anti-NRP1

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 21

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.