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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- BSM-52479R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Neuropilin-1 Monoclonal Antibody (49H9)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Positive Control: Human liver. Cellular Localization: Secreted. Selective granular cytoplasmic expression in liver with distinct positivity in plasma and extracellular matrix.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 49H9
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lane 1: Mouse heart; Lane 2: Mouse liver; Lane 3:Mouse kidney; Lane 4: Human liver; Probed with Neuropilin-1 (49H9)) Monoclonal Antibody (bsm-52479R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF(ICC) staining with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF(ICC) staining with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R)) at 1:50 dilution (red) compared with an unlabeled control (cells with out incubation with primary antibody; black).
