Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 690602 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#690602, RRID:AB_2629823
- Product name
- Purified anti-NFATC3
- Antibody type
- Monoclonal
- Description
- Nuclear factor of activated T cells C3 (NFATC3), also known as NFAT4 or NFATx, is a member of the NFAT family of transcription factors. Several NFATC3 variants generated by alternative splicing were identified. NFATC3 plays an important role in the regulation of T cell activation-related gene expression, especially in the induction of IL-2. NFATC3 is expressed in immune cells, such as double positive (DP) thymocytes, mature CD4+ T cells, and mast cells. It is also found in non-immune cells such as smooth muscle and Schwann cells. In the resting T cells, NFATC3 is located in the cytoplasm and is phosphorylated. Upon T cell activation by TCR stimulation, the increase of intracellular Ca2+ induces NFATC3 dephosphorylation via calcineurin. Dephosphorylated NFATC3 exposes the nuclear localization signal and is subsequently translocated from the cytosol to the nucleus. Through the highly conserved Rel-like binding domain, NFATC3 associates with specific consensus DNA sequences and regulates gene expression.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antibody clone number
- W15221A
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysate from human HeLa cells (lane 1), 293T cells (lane 2), and NIH-3T3 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-NFATC3 (clone W15221A) antibody. Proteins were visualized using an HRP goat anti-rat IgG antibody and chemiluminescence detection. Purified anti-β-actin (Clone Poly6221) antibody was used as a loading control.
- Conjugate
- Unconjugated