PAB11244

antibody from Abnova Corporation

Targeting: PIN1 dod

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB11244

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB11244, RRID:AB_1711115

Product name

PIN1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of PIN1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Aberrant expression of beta-catenin, Pin1 and cylin D1 in salivary adenoid cystic carcinoma: relation to tumor proliferation and metastasis.

Zhou CX, Gao Y
Oncology reports 2006 Sep;16(3):505-11

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The peptidyl-prolyl isomerase Pin1 regulates granulocyte-macrophage colony-stimulating factor mRNA stability in T lymphocytes.

Esnault S, Shen ZJ, Whitesel E, Malter JS
Journal of immunology (Baltimore, Md. : 1950) 2006 Nov 15;177(10):6999-7006

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Prolyl isomerase Pin1 expression predicts prognosis in patients with esophageal squamous cell carcinoma and correlates with cyclinD1 expression.

Fukuchi M, Fukai Y, Kimura H, Sohda M, Miyazaki T, Nakajima M, Masuda N, Tsukada K, Kato H, Kuwano H
International journal of oncology 2006 Aug;29(2):329-34

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using PIN1 polyclonal antibody (Cat # PAB11244) to detect endogenous PIN1 in HeLa whole cell lysates.The sample was run in duplicate.A band representing PIN1 is indicated by the arrowhead.Cell lysates were electrophoresed using a straight 15% polyacrylamide gel, followed by transfer to nitrocellulose.The membrane was probed with the primary antibody at a 1 : 700 dilution.A 1 : 5,000 dilution of HRP Gt-a-Rabbit IgG was used with a 15 sec exposure time.Personal Communication, L. D'agostino and A. Giordano, SHRO, Philadelphia, PA.