MA5-46896

antibody from Invitrogen Antibodies

Targeting: PRKCZ PKC2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-46896

Provider

Invitrogen Antibodies

Product name

Phospho-PKC zeta (Thr410) Monoclonal Antibody (HL1279)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Store as concentrated solution.

Antibody clone number

HL1279

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-PKC zeta (Thr410) in PKC proteins separated by 10% SDS-PAGE. Membrane was blotted with Phospho-PKC zeta (Thr410) Monoclonal antibody (Product # MA5-46896) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-PKC zeta (Thr410) in untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 µg) separated by 10% SDS-PAGE. Membrane was blotted with Phospho-PKC zeta (Thr410) Monoclonal antibody (Product # MA5-46896) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-PKC zeta (Thr410) in HeLa cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKC (pan) (zeta phospho Thr410) stained by PKC (pan) (zeta phospho Thr410) monoclonal antibody (Product # MA5-46896) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1,000. Blue: Fluoroshield with DAPI Scale bar= 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Phospho-PKC zeta (Thr410) in paraffin-embedded mouse cerebellum. Samples were incubated in Phospho-PKC zeta (Thr410) Monoclonal antibody (Product # MA5-46896). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.