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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA500823 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500823, RRID:AB_11126426
- Product name
- Anti-PPME1 mouse monoclonal antibody, clone OTI7F12 (formerly 7F12)
- Antibody type
- Monoclonal
- Description
- Anti-PPME1 mouse monoclonal antibody, clone OTI7F12 (formerly 7F12)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PPME1
- Isotype
- IgG
- Antibody clone number
- OTI7F12
- Vial size
- 100 µl
- Concentration
- 0.96 mg/ml
Submitted references Hydrogen peroxide redistributes the localization of protein phosphatase methylesterase 1.
Tang S, Lu C, Mo L, Wang X, Liang Z, Qin F, Liu Y, Liu Y, Huang H, Huang Y, Cai H, Xiao D, Guo S, Ouyang Y, Sun B, Li X
Life sciences 2018 Nov 15;213:166-173
Life sciences 2018 Nov 15;213:166-173
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (RC200009, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPME1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-PPME1 mouse monoclonal antibody (TA500823) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1(RC200009).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500823, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-PPME1 antibody(TA500823),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
- Validation comment
- FC
